Effect of Viral Infection on the Ultrastructural Organization of Black Currant Leaf Tissue Cells

One of the significant reserves for further increasing yield berry crops is to protect them from pests and diseases. Among latter, viral ones are especially dangerous. Therefore, methods virus diagnostics electron microscopy great importance, which makes it possible see particles, determine their shape, size, localization in tissues, identify anomalies affected plant cells. Objective. To conduct a comparative study healthy diseased blackcurrant leaves order degree influence two viruses on anatomical structure organelles inclusions, can be used diagnosis identifi cation affecting plants. Methods. The material was plants with symptoms reversion green speckles, detected visually when examining plantings this crop. morphology organelles, inclusions were studied using method ultrathin sections. Detected ultrastructural changes cells as diagnostic signs identifying viruses. Also, identified by external biological testing. Results. sections leaf tissue abnormal petals flower revealed bacillus-visible (Blackcurrant virus) Rabdoviridae family, easy due its large size appearance, differs similar features ordinary cellular components. Typical locations particles cytoplasm, nucleus, perinuclear zone. 271±7.19 nm long 78±2.31 diameter. According microscopic studying artificially infected Chenopodium quinoa, an inoculum leaves, virions Cucumis 1 Smith observed cells, freely located cytoplasm cell interspersed ribosomes. Individual areas high concentration also found parenchymal Zones surrounded double membrane differ saturation. When pathogen native preparations, had spherical shape smparticles showed that they average 29.6±0.59 nm. During ultrastructure 1. Smith, myelin-like bodies not only but extra-plasma space. Analysis chloroplasts speckles shows outgrowths cup-shaped formations present many Under various infections, there wide variety mitochondria’s shapes: elongated, cup- or club-like, etc. At same time, internal well. We at stage neurotization Nicotiana tabacum peroxisome matrix intensively filled crystalline have electron-dense surface system rods different configurations form rectangles trapezoids. They completely fill entire matrix. development pathology plants, destructive processes cover nucleus. As rule, takes lobed radially elongated shape. we studied, Blackcurrant reverse causes trait most. This may fact virus, accumulated quantities, exerts mechanical pressure nuclei thereby accelerates process deformation. A peculiar sign nucleus content particles. Of reversal most common nucleoplasm Conclusions. intracellular use energy systems components reproduction lead signifi cant morphological structural latter. In particular, studies comparison forms mitochondria, plastids modified under infl uence infection, namely Cucumber mosaic Chloroplasts noticeably swelled without existing starch grains, clumping absence gran thylakoids observed. Plastids highly reduced found. results shown species affiliation does cause specific mitochondria. Their transformations same: swelling, expansion crists decrease number, density matrix, so on. It chromatin small, interconnected granular lumps zones rather crystal peroxisome. If mosaics, spots caused pathogens transmission pathogen, advisable